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KMID : 0861020110260040133
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Korea Journal of Herbology
2011 Volume.26 No. 4 p.133 ~ p.137
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Protective effect of Socheongryong-Tang on hydrogen peroxide-induced hepatotoxicity
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Lee Ji-Seon
Oh Su-Young
Seo Sang-Hee
Kim Tae-Soo
Ma Jin-Yeul
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Abstract
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Objectives: Socheongryong-Tang (小靑龍湯, SCRT) has been widely used to treat respiratory disease. In this
study, we investigated the protective effects of SCRT on hydrogen peroxide-induced hepatotoxicity.
Methods: In the mouse primary liver cells, SCRT was pretreated for 1 h, and 1 mM H2O2 was treated to mouse
primary liver cells. Cell viability was analyzed by using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium
bromide (MTT) assay. Also, the activity of AST, ALT and LDH were measured for the evaluation the protective
effect of SCRT on H2O2-induced hepatotoxicity. Intracellular ROS level was analyzed by FACS.
Results: SCRT pretreatment decreased H2O2-induced hepatotoxicity and intracellular ROS production.
Pretreatment of SCRT significantly reduced the cytotoxic effect induced by H2O2, associated with reducing DNA
fragmentation and AST, ALT, LDH activities.
Conclusions: These results suggest that SCRT has protective effect against H2O2-induced hepatotoxicity.
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KEYWORD
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Socheongryong-Tang, , Hepatotoxicity, ROS
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